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cytometry data analysis software  (Becton Dickinson)
90
Becton Dickinson cytometry data analysis software
Iva- or Aib containing peptides activate T cells. Cell surface expression of CD25 molecules on OT-I cells following stimulation with peptide antigens. ( A – G ) representative flow <t>cytometry</t> dot plots show CD8 T cell activation after 24 h stimulated with either the native ova-control peptides (( A ): short Ova 257-264 (SIINFEKL) or ( B ): longer Ova 248-269 ), unstimulated ( C ) or with the ova peptides substituted with exo amino acids ( D ): Ova 248-269 (Iva) and ( F ): Ova 248-269 (Aib) or peptides substituted natural amino acids ( E ): Ova 248-269 (Val) and ( G ): Ova 248-269 (Ala); ( H ) frequency of CD25 positive single CD8 cells from three technical replicas of three biological replicas. Activation of Iva-peptides and Aib-peptide was significantly reduced compared to the native Ova peptide control (( B , H ) p -value < 0.0001) or the peptides substituted with Val (( E , H ) 24 h and 72 h: p -value < 0.0001; 48 h: p -value 0.0003) or Ala (( G , H ) 24 h: p -value 0.0009; 48 h and 72 h: p -value < 0.0001).
Cytometry Data Analysis Software, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Iva- or Aib containing peptides activate T cells. Cell surface expression of CD25 molecules on OT-I cells following stimulation with peptide antigens. ( A – G ) representative flow cytometry dot plots show CD8 T cell activation after 24 h stimulated with either the native ova-control peptides (( A ): short Ova 257-264 (SIINFEKL) or ( B ): longer Ova 248-269 ), unstimulated ( C ) or with the ova peptides substituted with exo amino acids ( D ): Ova 248-269 (Iva) and ( F ): Ova 248-269 (Aib) or peptides substituted natural amino acids ( E ): Ova 248-269 (Val) and ( G ): Ova 248-269 (Ala); ( H ) frequency of CD25 positive single CD8 cells from three technical replicas of three biological replicas. Activation of Iva-peptides and Aib-peptide was significantly reduced compared to the native Ova peptide control (( B , H ) p -value < 0.0001) or the peptides substituted with Val (( E , H ) 24 h and 72 h: p -value < 0.0001; 48 h: p -value 0.0003) or Ala (( G , H ) 24 h: p -value 0.0009; 48 h and 72 h: p -value < 0.0001).

Journal: Microorganisms

Article Title: A Weakened Immune Response to Synthetic Exo-Peptides Predicts a Potential Biosecurity Risk in the Retrieval of Exo-Microorganisms

doi: 10.3390/microorganisms8071066

Figure Lengend Snippet: Iva- or Aib containing peptides activate T cells. Cell surface expression of CD25 molecules on OT-I cells following stimulation with peptide antigens. ( A – G ) representative flow cytometry dot plots show CD8 T cell activation after 24 h stimulated with either the native ova-control peptides (( A ): short Ova 257-264 (SIINFEKL) or ( B ): longer Ova 248-269 ), unstimulated ( C ) or with the ova peptides substituted with exo amino acids ( D ): Ova 248-269 (Iva) and ( F ): Ova 248-269 (Aib) or peptides substituted natural amino acids ( E ): Ova 248-269 (Val) and ( G ): Ova 248-269 (Ala); ( H ) frequency of CD25 positive single CD8 cells from three technical replicas of three biological replicas. Activation of Iva-peptides and Aib-peptide was significantly reduced compared to the native Ova peptide control (( B , H ) p -value < 0.0001) or the peptides substituted with Val (( E , H ) 24 h and 72 h: p -value < 0.0001; 48 h: p -value 0.0003) or Ala (( G , H ) 24 h: p -value 0.0009; 48 h and 72 h: p -value < 0.0001).

Article Snippet: For the analysis, FlowJo cytometry data analysis software (BD Bioscience) was used and the significance of the difference between the means of two data set (student t -test) was determined with GraphPad Prism scientific 2D graphing and statistics software.

Techniques: Expressing, Flow Cytometry, Activation Assay